Modulatory Effect of Fenugreek Loaded PLGA Nanoparticles on Lipofuscinogenesis in Pancreas of Alloxan Induced Diabetic Mice

Many traditional treatments have been recommended in the alterative system of medicine for the diabetes mellitus. Current research is now directed towards finding naturally occurring antidiabetic properties from plant origin. In Indian system of medicine Trigonella foenum graecum is an important medicinal plant and its leaves and seeds have been used in various ailments and as a health tonic. The purpose of this study was to examine the antioxidant activity of fenugreek seed extract (FSE) and its nanoparticles (FNPs) on body weight, pancreatic gland weight, lipid peroxidation and fluorescence product of the pancreas of alloxan induced diabetic mice. Adult albino male mice (Mus musculus L) were divided into four group’s viz. i. Control Group: male mice were given subcutaneous injection of 0.15M acetate buffer pH 5.4 for 15 days. ii. Diabetic Groupmice were given single subcutaneous injection of alloxan 150 mg/kg body weight. iii. Diabetic → FSE: mice were given subcutaneous injection of FSE at a dose of 15mg/ kg body weight to diabetic mice for 15 days. iv. Diabetic → FNPs: mice were given subcutaneous injection of FNPs at a dose of 15mg/ kg body weight to diabetic mice for 15 days. Body weight and pancreatic gland weight was reduced in diabetic group but increased in fenugreek supplementary group. The end product of lipid peroxidation malondialdehyde (MDA) and fluorescence product were increased in diabetic group and after fenugreek administration the level of both the parameters reduced significantly. The results suggest that an anti-lipid peroxidation activity of fenugreek seeds and its nanoparticles, nanoparticles treated group showed better results than fenugreek seed extract treated groups indicating that the fenugreek nanoparticles are best antidiabetic and antioxidant as compared fenugreek seed extract.